817500

TSKgel^® Octadecyl-2PW (5 µm) HPLC Columns

L x I.D. 15 cm × 4.6 mm, C18 (octadecyl) phase

• 同義詞: TSKgel^® Reversed Phase HPLC Column (Polymer-based)
• 分類程式碼代碼: 41115711
• eCl@ss: 32110501

屬性

• product name: TSKgel^® Octadecyl-2PW HPLC Column, phase C18 (octadecyl), L × I.D. 15 cm × 4.6 mm, 5 μm particle size
• 材料: stainless steel hardware
• 產品線: TSKgel^®
• 製造商／商標名: Tosoh 17500
• 標籤範圍: 1 meq/mL ligand density (estimate)
• 參數: 0-100% organic solvent; 1.2 mL/min flow rate; 120 bar max. pressure; 4-50 °C temperature
• 技術: HPLC: suitable
• 長度 × 內徑: 15 cm × 4.6 mm
• 基質: polymer particle platform
• 基質活性組: C18 (octadecyl) phase
• 粒徑: 5 μm
• 孔徑: 125 Å
• 工作pH值: 2-12
• 分離技術: reversed phase

描述

一般說明

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

外觀

Shipped in 55% acetonitrile - 45% water.

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法律資訊

TSKgel is a registered trademark of Tosoh Corporation