814449

TSKgel^® Boronate-5PW (10 µm) HPLC Columns

L x I.D. 5 cm × 5 mm, m-aminophenyl boronate phase

• 同義詞: TSKgel^® Boronate-5PW HPLC Column
• 分類程式碼代碼: 41115711

屬性

• 產品名稱: TSKgel^® Boronate-5PW HPLC Column, phase m-aminophenyl boronate, L × I.D. 5 cm × 5 mm, 10 μm particle size, glass
• 材料: glass
• 產品線: TSKgel^®
• 分析物化學類別: proteins
• 製造商／商標名: Tosoh 14449
• 參數: ≤20% organic solvent; ≤3 M salt concentration; >0 °C temperature; 1.2 mL/min flow rate; 30 bar max. pressure
• 技術: HPLC: suitable
• 長度 × 內徑: 5 cm × 5 mm
• 基質: hydroxylated methacrylic polymer
• 基質活性組: m-aminophenyl boronate phase
• 粒徑: 10 μm
• 工作pH值: 2-9
• 容量: 40 μmol/mL, resin adsorption capacity (sorbitol)
• 分離技術: affinity
• 儲存溫度: 2-8°C

描述

一般說明

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

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法律資訊

TSKgel is a registered trademark of Tosoh Corporation