技術
HPLC: suitable
測試參數
column: TSKgel DEAE-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (with 5 mm DEAE-NPR guard column) (813075)
mobile phase: [A] 1 M sodium chloride/25 mm Tris-HCl, pH 9.0; [B] 25 mM Tris-HCl, pH 9.0
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 260 nm
injection: 20 μL of 50 μg/mL Hae III digest of pBR322 DNA
適合性
application for HPLC
應用
life science and biopharma
法律資訊
TSKgel is a registered trademark of Tosoh Corporation
分析物
描述
pBR322 Hae III Digest, lyophilized powder
Rapid separation, quantitation, and purification of products of polymerase chain reaction by liquid chromatography
Elena D. Katz, Lawrence A. Haff, Roy Eksteen
Journal of Chromatography A, 512, 433-444 (1990)
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