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Key Documents

581512-U

Supelco

Ascentis® Si (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Si HPLC 色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

材料

stainless steel column

品質等級

100

agency

suitable for USP L3

產品線

Ascentis®

特點

endcapped: no

製造商／商標名

Ascentis®

包裝

1 ea of

參數

≤70 °C temp. range
400 bar pressure (5801 psi)

技術

HPLC: suitable
LC/MS: suitable

長度 × 內徑

15 cm × 4.6 mm

表面積

450 m2/g

雜質

<5 ppm metals

基質

fully porous particle
silica gel high purity, spherical

基質活性組

silica phase

粒徑

5 μm

孔徑

100 Å

工作pH值範圍

2-6

應用

food and beverages

分離技術

hydrophilic interaction (HILIC)
normal phase

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一般說明

Ascentis 系列色谱柱是 Supelco 科学家推出的第四代 HPLC 色谱柱技术。Ascentis 系列色谱柱是以高纯硅胶为基质，孔径 100Å，硅胶粒径为 3、5 和 10 微米。该色谱柱用于小分子的应用，柱内径可从微径柱（1.0mm 内径）扩展到制备尺寸（50mm 内径）。该系列包括 C18、C8、苯基、Si 和内嵌极性基团相（反相酰胺）。

Ascentis Si 是具有高载样量和优良峰形的硅胶柱。Ascentis Si 在正相和 HILIC/ANP（水性正相）模式下都可使用。

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法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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分析證明 (COA)

Lot/Batch Number
275720-04
275720-03
275720-02
275720-01
275623-04

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Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
Luana Magalhães et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1008, 98-107 (2015-12-08)
Nucleoside triphosphate diphosphohydrolase (NTPDase) is an enzyme belonging to the apyrase family that participates in the hydrolysis of the nucleosides di- and triphosphate to the corresponding nucleoside monophosphate. This enzyme underlies the virulence of parasites such as Leishmania. Recently, an
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文章

HPLC Analysis of Sugars on Ascentis® Si

Separation of Sucrose; Maltose; Maltotriose

條款

Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography

Polar Compound Retention using Aqueous Normal-Phase (ANP/HILIC) Chromatography

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si

Separation of (±)-α-Tocopherol, analytical standard; Menadione (K3), analytical standard; (+)-γ-Tocopherol, analytical standard; Cholecalciferol (D3), analytical standard

Chromatograms

application for HPLCapplication for HPLCapplication for HPLC

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