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575656-U

Supelco

HybridSPE®-Phospholipid 96-well solid phase extraction (SPE) Plate

Volume 2 mL, bed wt., 50 mg, pk of 1

同義詞:

HybridSPE (phospholipid and protein removal) 96-well plate, 50 mg

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About This Item

分類程式碼代碼:
41104018
NACRES:
NB.21

產品名稱

HybridSPE ®-磷脂SPE小柱, 96-well Plate, bed wt. 50 mg, volume 2 mL, pk of 1

品質等級

產品線

HybridSPE®

形狀

solid

成份

bed wt., 50 mg

包裝

pk of 1

技術

solid phase extraction (SPE): suitable

容積

2 mL

基質活性組

zirconia-based phase

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一般說明

HybridSPE-Phospholipid磷脂去除技术是一种简单且通用的样本制备平台,设计用于在 LC-MS 或 LC-MS/MS 分析之前,从生物血浆和血清中总体去除内源性蛋白和磷脂干扰。首先通过加入和混合酸化乙腈使生物血浆或血清发生蛋白沉淀。然后通过离心除去沉淀蛋白,并将所得上清液上样至 HybridSPE-Phospholipid 96 孔板或固相萃取柱上,其作为化学过滤器,专门靶向去除内源性样品磷脂。截留磷脂的原理是HybridSPE-Phospholipid固定相键合的专有氧化锆离子和所有磷脂都具有的磷酸根发生高度选择性的路易斯酸碱相互作用。得到的洗脱液可立即用于 LC-MS 或 LC-MS-MS 分析。

HybridSPE-Phospholipid 96 孔板和Ultra固相萃取柱有“孔内”/“柱内”沉淀法可供选择。即先将生物血浆/血清加入孔内或柱内,接着加入酸化乙腈(沉淀剂)。短暂混合/涡旋后,抽真空。96孔板和 Ultra固相萃取柱含有一系列低孔隙率疏水滤膜/筛板,这些填充柱床的滤膜/筛板组件作为深层过滤器,有助于在提取过程中同时去除磷脂和沉淀蛋白。标准 HybridSPE-Phospholipid磷脂固相萃取小柱需要采用“离柱”沉淀法。

應用


  • Rapid analysis of 65 pharmaceuticals and 7 personal care products in plasma and whole-body tissue samples of fish using acidic extraction, zirconia-coated silica cleanup, and liquid chromatography-tandem mass spectrometry: This study discusses the advanced application of chromatography and mass spectrometry, which could involve the use of technologies like HybridSPE®-Phospholipid for sample preparation, specifically for rapid analysis in biological matrices (Tanoue et al., 2020).

  • Multi LC-MS/MS and LC-HRMS Methods for Determination of 24 Mycotoxins including Major Phase I and II Biomarker Metabolites in Biological Matrices from Pigs and Broiler Chickens: Demonstrates how LC-MS/MS, potentially incorporating HybridSPE®-Phospholipid technology for sample cleanup, facilitates the sensitive and accurate detection of mycotoxins in complex biological samples (Lauwers et al., 2019).

  • Evaluation of an Ion Trap Toxtyper Liquid Chromatography With An Ion Trap Mass Spectrometric Instrument (Toxtyper) for Drug of Abuse Screening in Oral Fluid: Explores the utility of specific LC-MS/MS methodologies which could integrate HybridSPE®-Phospholipid for bioanalytical sample cleanup, enhancing drug screening accuracy (Plecko et al., 2018).

  • Effective phospholipid removal from plasma samples by solid phase extraction with the use of copper (II) modified silica gel cartridges: This research potentially aligns with the use of HybridSPE®-Phospholipid technologies for phospholipid removal, focusing on enhancing the purity and quality of chromatography samples (Flieger et al., 2017).

  • Liquid chromatography mass spectrometry determination of perfluoroalkyl acids in environmental solid extracts after phospholipid removal and on-line turbulent flow chromatography purification: Illustrates a specialized application of chromatography and mass spectrometry where HybridSPE®-Phospholipid could be applied to improve the analysis of environmental samples by efficient phospholipid removal (Mazzoni et al., 2016).

特點和優勢

  • 融合了蛋白质沉淀的简单性和固相萃取(SPE)定向除去磷脂的选择性
  • 通过完全除去磷脂和沉淀蛋白,减少离子抑制
  • 2-3 步通用流程
  • 方法开发最少,甚至不需要方法开发
  • 有 96 孔和 1 mL 萃取柱规格可供选择

法律資訊

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

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條款

his study demonstrates the analysis of Warfarin in plasma samples utilizing chiral and achiral (reversed-phase) LC-MS and effective sample prep to remove endogenous phospholipids

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

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