product name
Discovery® HS C18 高效液相色谱柱, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
材料
stainless steel column
agency
suitable for USP L1
產品線
Discovery®
特點
endcapped
製造商/商標名
Discovery®
包裝
1 ea of
標籤範圍
20% Carbon loading
參數
≤70 °C temp. range
400 bar pressure (5801 psi)
技術
HPLC: suitable
LC/MS: suitable
長度 × 內徑
15 cm × 2.1 mm
表面積
300 m2/g
表面覆盖率
3.2 μmol/m2
雜質
<10 ppm metals
基質
silica gel, high purity, spherical particle platform
fully porous particle
基質活性組
C18 (octadecyl) phase
粒徑
5 μm
孔徑
120 Å
工作pH值範圍
2-8
應用
food and beverages
分離技術
reversed phase
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文章
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
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