product name
Ascentis® Express F5, 2 μm UHPLC 色谱柱, 2 μm particle size, L × I.D. 7.5 cm × 3 mm
材料
stainless steel column
agency
suitable for USP L43
產品線
Ascentis®
特點
endcapped
製造商／商標名
Ascentis®
包裝
1 ea of
參數
1000 bar max. pressure (14500 psi)
60 °C temp. range
技術
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
長度 × 內徑
7.5 cm × 3 mm
表面積
120 m2/g
雜質
<5 ppm metals
基質
Fused-Core particle platform
superficially porous particle
基質活性組
PFP (pentafluorophenyl) phase
粒徑
2 μm
孔徑
90 Å pore size
工作pH值
2-8
應用
food and beverages
分離技術
reversed phase
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一般說明
Ascentis® Express 2.0 μm F5是基于Fused-Core®颗粒设计的快速UHPLC柱。这种颗粒设计在0.4 μm厚的多孔壳中的扩散路径较浅，且总体粒径小至2 μm，因此柱效很高。Ascentis® Express 2.0 μm F5的键合封端的二甲基五氟苯基丙基固定相由于存在负电性氟而可提供含缺电子苯环的稳定的反相填充。除了形成π-π相互作用和温和的空间相互作用外，F5相还通过极性相互作用保留化合物。F5相具有极性和非极性特性，可表现出双模式保留行为，有时会随流动相中乙腈含量的变化而产生“ U形”保留，在ACN浓度低和高的情况下保留都会增加（ 反相和HILIC保留模式）。Ascentis® Express 2.0 μm F5可作为C18选择性的补充，对碱性、酸性或中性化合物进行分析。
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 120, 112-119 (2016-01-01)
A new high-performance liquid chromatography method using fused-core column for fast separation of resveratrol and polydatin has been developed and used for quality control of nutraceuticals with resveratrol and polydatin content. Retention characteristics (log k) were studied under different conditions
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