ZRB2800

Anti-Collectin-11 Antibody, clone 1I5-H2 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: CL-11, CL-K1, Collectin kidney protein 1
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: recombinant monoclonal
• 描述: clone 1I5-H2 ; recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 28.67 kDa; observed mol wt ~35 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 25
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_076932
• UniProt登錄號: Q9BWP8
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... COLEC11(78989)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1I5-H2 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Collectin-11. It targets an epitope within 17 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human Collectin-11.

應用

Quality Control Testing

Evaluated by Western Blotting in Human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Collectin-11 in Human liver tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collectin-11 in Human kidney tissue sections.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Collectin-11 in Human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Collectin-11 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Collectin-11 (UniProt: Q9BWP8; also known as Collectin kidney protein 1, CL-K1) is encoded by the COLEC11 gene (also known as UNQ596/PRO1182) gene (Gene ID: 78989) in human. Collectin-11, a disulfide-linked, calcium-dependent, homotrimeric protein of the lectin family of pattern recognition molecules, is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature protein. It contains a collagen-like domain (aa 65-112) and a C-type lectin domain (aa 149-265). It is also reported to form heteromeric complexes with Collectin Liver 1 (CL-10) in the serum. Collectin-11 is most obviously expressed in renal tubules by epithelial cells. It displays antimicrobial properties and has ability to trigger complement activation via the lectin pathway. It plays a role in innate immunity through its ability to bind non-self-sugars presented by microorganisms and to activate the complement through the recruitment of Mannan-binding lectin-associated serine protease 1 (MASP-1). Its structure is similar to other C-type lectins and consists of a globular head followed by a neck and a collagenous tail. The head region contains a carbohydrate recognition domain (CRD), and the tail contains binding sites for MASPs. Collectin-11 monomers form a triplet structure that self-combines to form oligomers with higher avidity of binding to ligand. Ten isoforms of Collectin-11 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nauser, CL., et al. (2018). Front. Immunol. 9; 2023; Hansen, S., et al (2010). J. Immunol.185(10); 6096-6104; Keshi, H., et al. (2006). Microbiol Immunol. 50(12); 1001-13).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.