ZRB2665

Anti-TGF-β1 Antibody, clone 4E3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: TGFB, Transforming growth factor beta-1, Transforming growth factor beta-1 proprotein
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 4E3, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 44.33 kDa; observed mol wt ~13 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: horse, bovine, monkey, porcine, rat, nonhuman primates, goat, sheep
• 包裝: antibody small pack of 25
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgGκ
• 表位序列: C-terminal
• Protein ID登錄號: NP_000651
• UniProt登錄號: P01137
• 環保替代類別: Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... TGFB1(7040)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 4E3 is a ZooMAb^® recombinant rabbit monoclonal antibody that specifically detects TGF-beta-1. It targets an epitope within 18 amino acids from the C-terminal region of the protein.

免疫原

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of human TGF- β1.

應用

Quality Control Testing

Evaluated by Western Blotting in TGF-β1 recombinant protein.

Western Blotting Analysis: A 1:50,000 dilution from a representative lot detected TGF-β1 in recombinant fragment from human TGF-β1 protein .

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TGF-β1 in HEK-293 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TGF-β1 in human bone marrow and human stomach tissue sections.

Flow Cytometry Analysis (FC): 1 µg from a representative lot detected TGF-β1 in one million HEK-293 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Transforming growth factor beta-1 proprotein (UniProt: P01137) is encoded by the TGFB1 (also known as TGFB) gene (Gene ID: 7040) in human. Transforming growth factor beta-1 proprotein is synthesized with a signal peptide (aa 1-29) and is cleaved in the Golgi apparatus by FURIN to form two chains; Latency-associated peptide (LAP) (aa 30-278) and Transforming growth factor beta-1 (TGF-beta-1) (aa 279-390), which remain non-covalently linked, rendering TGF-beta-1 inactive. These 2 chains, LAP and TGF-beta-1 constitutes the regulatory and active subunit of TGF-beta-1, respectively. TGFB1 belongs to the TGF-β superfamily of secreted proteins (primarily localized in the extracellular matrix once secreted) and is involved in a variety of signaling pathways that involve serine/threonine protein kinases. It is widely expressed but tightly regulated at both the transcriptional and post-transcriptional levels. It is produced by many cell types, including macrophages, endothelial cells, fibroblasts, and epithelial cells and is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGFB1 is among the most potent fibrogenic cytokines inducing organ fibrosis by promoting the formation of myofibroblasts. TGFB1 is a key player in the development of fibrotic diseases such as pulmonary fibrosis, liver cirrhosis, and kidney fibrosis, where it stimulates the deposition of extracellular matrix components, leading to tissue scarring. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable