ZRB2408

Anti-CCL20/MIP-3α Antibody, clone 1F5-H1 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Beta-chemokine exodus-1, C-C motif chemokine 20, CC chemokine LARC, Liver and activation-regulated chemokine, MIP-3-alpha, Macrophage inflammatory protein 3 alpha, Small-inducible cytokine A20
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1F5-H1, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_004582
• UniProt登錄號: P78556
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... CCL20(6364)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1F5-H1 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects C-C motif chemokine 20 (CCL20). It targets an epitope within 16 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human C-C motif chemokine 20 (CCL20).

應用

Quality Control Testing

Evaluated by Western Blotting using recombinant Human CCL20/MIP3 .

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant Human CCL20/MIP-3 .

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CCL20/MIP-3 in HepG2 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CCL20/MIP-3 in Human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Human CCL20/MIP-3 protein with a KD of 1.3 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected CCL20/MIP-3 in CCL20/MIP-3 .

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

C-C motif chemokine 20 (UniProt: P78556; also known as Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, Small-inducible cytokine A20) is encoded by the CCL20 (also known as LARC, MIP3A, SCYA20) gene (Gene ID: 6364) in human. CCL20 is a secreted protein that is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to produce the mature protein. The mature CCL20 can be further cleaved into 1-67, 1-64, and 2-70 fragments. It is expressed in a variety of human tissues and by a large number of immune cells. It is induced by bacterial lipopolysaccharides, TNFα, and Interferon-γ. It serves as a ligand for C-C-chemokine receptor 6 (CCR6). Its binding o CCR6 induces a strong chemotactic response and mobilization of intracellular calcium ions. CCL20-CCR6 pair is responsible for chemotaxis of dendritic cells, effector/memory T-cells and B-cells. CCL20 is reported to bind to a shallow extracellular pocket of CCR6 where the N-terminal tyrosine 27 and leucine 38 of CCR6 wrap onto the globular core of CCL20. It is shown to be highly active on resting memory T cells, indicating that it may be important for their recruitment to inflammatory sites early in an immune response. CCL20 is also involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation and is required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Wasilko, JD., et al. (2020). Nat. Commun. 11; Article 3031; Lee, AYS., et al. (2013). Front. Immunol. 4; 194; Schutyser, E., et al. (2000). J. Immunol. 165(8); 4470-4477).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable