ZRB2377

Anti-p-PKCβ (Ser660) Antibody, clone 2C11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Anti-Ly-6C1 Antibody, clone 2H5 ZooMAb^® Rabbit Monoclonal, EC:2.7.11.13, PKC-B, PKC-beta, Protein kinase C beta type
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2C11, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 76.87 kDa; observed mol wt ~77 kDa
• 純化經由: using Protein A
• 物種活性: mouse, monkey
• 物種活性（以同源性預測）: rat, rabbit, bovine, human
• 包裝: antibody small pack of 25
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_997700
• UniProt登錄號: P05771
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... PRKCB(5579)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2C11 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Protein Kinase C β (PKCβ) phosphorylated on serine 660.

免疫原

KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserine 660 from the C-terminal region of human Protein Kinase C β (PKCβ).

應用

Quality Control Testing

Evaluated by Western Blotting in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA).

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKC β phosphorylated on serine 660 in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/mL; 20 min).

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound p-PKC β (Ser660) peptide with a KD of 9.8 x 10-7 in an affinity binding assay.

Peptide Inhibition Assay: Target band detection in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/mL; 20 min) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-PKCβ (Ser660) in serum starved NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/ml) for 20 min.).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Protein kinase C beta type (UniProt: P05771; also known as EC:2.7.11.13, PKC-B, PKC-beta) is encoded by the PRKCB (also known as PKCB, PRKCB1) gene (Gene ID: 5579) in human. PKCβ is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine kinase that is involved in multiple cellular processes, including the regulation of B-cell receptor, oxidative stress-induced apoptosis, insulin signaling, and endothelial cell proliferation. PKCβ is comprised of two splice variants, PKCβI and PKCβII, which are from the same gene and differ only in their last 50 amino acids. It binds three calcium ions per subunit and these ions are bound to the C2 domain (aa 158-275). Three specific sites: threonine 500 in the activation loop of the kinase domain; threonine 642 in the turn motif, and serine 661 in the hydrophobic region need to be phosphorylated for its full activation. Phosphorylation on threonine 500 renders it competent for autophosphorylation. Subsequent phosph

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable