ZRB2313

Anti-Chk1 Antibody, clone 2E14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Serine/threonine-protein kinase Chk1;EC:2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2E14, recombinant monoclonal
• 描述: 2E14 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 54.43 kDa; observed mol wt ~60 kDa
• 純化經由: using Protein A
• 物種活性: human, mouse
• 物種活性（以同源性預測）: canine, porcine, monkey, feline
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: Internal
• Protein ID登錄號: NP_001107593
• UniProt登錄號: O14757
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CHEK1(1111)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2E14 is a ZooMAb^® recombinant monoclonal antibody that specifically detects Checkpoint kinase-1 (Chk1). It targets an epitope within 21 amino acids from the internal region.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Checkpoint kinase-1 (Chk1).

應用

Quality Control Testing

Evaluated by Western Blotting in Panc-1 cell lysates.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Chk1 in Panc-1 cell lysates.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Chk1 in lysates from HepG2 and Mouse pancreatic β cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Chk1 in A431 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Chk1 in human breast cancer and human colon cancer.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Serine/threonine-protein kinase Chk1 (UniProt: O14757; also known as EC:2.7.11.1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1) is encoded by the CHEK1 (also known as CHK1) gene (Gene ID: 1111) in human. Chk1 is a serine/threonine-protein kinase that is required for checkpoint-mediated cell cycle arrest. It is expressed ubiquitously, and is abundantly expressed in thymus, testis, small intestine, and colon. It is activated through phosphorylation predominantly by ATR but also by ATM in response to DNA damage or inhibition of DNA replication. It may negatively regulate cell cycle progression during unperturbed cell cycles that is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Chk1 recognizes the substrate consensus sequence [R-X-X-S/T]. It binds to and phosphorylates CDC25A (at Ser 178, Thr 507) and CDC25C (at Ser 216). These phosphorylations create binding sies for 14-3-3- proteins, which inhibit CDC2A and CDC2C. Phosphorylation of Chk1 on serine 345 induces a change in the conformation of the protein that activates the kinase activity and is shown to be a prerequisite for interaction with FBXO6 and subsequent ubiquitination at lysine 436. Phosphorylation at serine 345 increases its binding to 14-3-3 proteins and promotes its nuclear retention. Conversely, dephosphorylation at serine 345 by protein phosphatase, Mg2+/Mn2+ dependent 1D (PPM1D) contributes to exit from checkpoint mediated cell cycle arrest. The activated, serine 345 phosphorylated form can undergo polyubiquitination at lysine 436 and undergo degradation ensuring  that activated Chk1 does not accumulate as cells progress through S phase. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Moiseeva, TN., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(27); 13374-13383; Chen, MS., et al. (2003). Mol. Cell Biol. 23(21); 7488-7497; Feijoo, C., et al. (2001). J. Cell. Biol. 154(5); 913-923).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable