ZRB2165

Anti-PRRX1 Antibody, clone 1L16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Paired mesoderm homeobox protein 1;Homeobox protein PHOX1;Paired-related homeobox protein 1;PRX-1
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1L16, recombinant monoclonal
• 描述: 1L16 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 24.3 kDa; observed mol wt ~30-33 kDa
• 純化經由: using Protein A
• 物種活性: rat, human
• 物種活性（以同源性預測）: mouse
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_073207
• UniProt登錄號: P54821
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PRRX1(5396)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1L16 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Paired mesoderm homeobox protein 1 (PRRX1). It targets an epitope within 21 amino acids from the N-terminal half. Predicted to react with both isoforms.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Paired mesoderm homeobox protein 1 (PRRX1).

應用

Quality Control Testing

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: 1:10,000 of this antibody detected PRRX1 in C6 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PRRX1 in C6 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PRRX1 in human testis tissue sections.

Western Blotting Analysis: A representative lot detected PRRX1 in Western Blotting applications (Data Courtesy of Dr Takarada from Okayama University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Paired mesoderm homeobox protein 1 (UniProt: P54821; also known as Homeobox protein PHOX1, Paired-related homeobox protein 1, PRX-1) is encoded by the PRRX1 (also known as PMX1) gene (Gene ID: 5396) in human. PRRX1 is considered as a master transcription factor in mesenchymal cells in embryos and in certain cancer types. It can serve as a transcription co-activator, enhancing the DNA-binding activity of serum response factor that is required for the induction of genes by growth and differentiation factors. PRRX1 acts as a transcriptional regulator of muscle creatine kinase and has a role in the establishment of diverse mesodermal muscle types. It binds to an A/T-rich element in the muscle creatine enhancer. PRRX1 is reported to orchestrates the functional drift of fibroblasts into myofibroblastic phenotype via TGF-β signaling. These reprogrammed fibroblasts have myofibroblastic functions that result in enhanced tumorigenicity and aggressiveness of cancer. PRRX1 expression in cancer-associated fibroblast (CAF) is reported to have an unfavorable prognosis in multiple cancer types. Fibroblast-specific PRRX1 depletion can induce long-term and sustained complete remission of chemotherapy-resistant cancer in murine models. PRRX1 expression is also observed in quiescent human oligodendrocyte progenitor cells (OPCs) where it induces cell-cycle arrest and inhibits migration. Enforced PRRX1 expression can result in reversible G1/0 arrest. Two isoforms of PRRX1 have been described that are produced by alternative splicing. Although both isoforms are known to reduce OPCs proliferation, only PRRX1a reduces migration. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lee, KW., et al. (2022). Nat. Commun. 13; Article 2793; Wang, J., et al. (2018). Cell Reports 25; 3435-3450).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1