ZRB1904

Anti-Fyn Antibody, clone 2F15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2F15, recombinant monoclonal
• 描述: 2F15 Clone
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 60.76 kDa; observed mol wt ~56 kDa
• 純化經由: using Protein A
• 物種活性: rat, mouse, human
• 物種活性（以同源性預測）: canine, porcine, bovine, monkey, feline
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal
• Protein ID登錄號: NP_001357458 and NP_694592
• UniProt登錄號: P06241
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FYN(2534)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2F15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase Fyn. It targets an epitope within 20 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Tyrosine-protein kinase Fyn.

應用

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Fyn in Jurkat cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fyn in Ramos, C2C12, MOLT4, and PC12 cell lysates.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Fyn in one million Jurkat cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Fyn in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Fyn in Jurkat cells.

Affinity Binding Assay: A representative lot of this antibody bound Fyn with a KD of 3.6 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Tyrosine-protein kinase Fyn (UniProt: P06241; also known as EC:2.7.10.2, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, SLK, p59-Fyn) is encoded by the FYN gene (Gene ID: 2534) in human. Fyn is a non-receptor tyrosine-protein kinase that plays a role in the regulation of cell growth and survival, cell adhesion, integrin-mediated signal transduction, cytoskeletal remodeling, cell motility, immune response, and axon guidance. It is present in lipid rafts and its palmitoylation is shown to be crucial for proper trafficking. Fyn plays a role in insulin sensitivity and lipid utilization by regulating LKB1 function and FYN knockout mice display significant reductions in adiposity, fasting glucose and insulin levels, and improved insulin sensitivity. FYN deletion improves plasma and tissue triglycerides, increases energy expenditure, and enhances fatty acid oxidation. Its protein kinase domain is localized in amino acids 271-534. It regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator Wiskott Aldrich syndrome (WAS) protein and the microtubule-associated proteins MAP2 and MAPT. Its phosphorylation on Tyr 531 in the C-terminal region by C-terminal Src kinase (CSK) maintains it an inactive state and its dephosphorylation by PTPRC/CD45 leads to its activation. It undergoes autophosphorylation at Tyr 420 and dephosphorylation at this site is shown to negatively regulate T- cell receptor signal transduction. Three isoforms of Fyn have been described that are produced by alternative splicing. Isoform 1 is highly expressed in the brain and isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. Isoform 3 lacks amino acids 233-287. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wiede, F., et al. (2011). J. Clin. Invest. 121(12); 4758-4774; Mustelin, T., et al. (1992). Eur. J. Immunol. 22(5); 1173-1178).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable