ZRB1776

Anti-Syk Antibody, clone 2G22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Tyrosine-protein kinase SYK;EC:2.7.10.2;Spleen tyrosine kinase;p72-Syk
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2G22, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 72.07 kDa; observed mol wt ~70 kDa
• 純化經由: using Protein A
• 物種活性: human, mouse, rat
• 物種活性（以同源性預測）: canine, feline, porcine, bovine, monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_001167638
• UniProt登錄號: P43405
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SYK(6850)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2G22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Syk. It targets an epitope within 16 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Tyrosine-protein kinase SYK (Syk).

應用

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Syk in Ramos cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Syk in lysates from A431 and Raw264.7 cells and Mouse Spleen tissue.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Syk in human tonsil tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected Syk in one million Ramos cells.

Affinity Binding Assay: A representative lot of this antibody bound Syk with a KD of 6.6 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Tyrosine-protein kinase SYK (UniProt: P43405; also known as EC:2.7.10.2, Spleen tyrosine kinase, p72-Syk) is encoded by the SYK gene (Gene ID: 6850) in human. Syk is a non-receptor tyrosine kinase that is highly expressed in hematopoietic cells. It plays a crucial role not only in adaptive immune receptor signaling, but also in cellular adhesion, innate immune recognition, osteoclast maturation, platelet activation and vascular development. Syk contains two SH2 domains (aa 15-107 and 168-259) that mediate its interaction with the phosphorylated ITAM domains of transmembrane proteins. Its protein kinase domain is localized in amino acids 371-631. Intramolecular binding of the interdomains A and B (aa 108-160 and 260-370) to parts of its catalytic domain keeps the catalytic center in an inactive conformation. The phosphorylation of the interdomains or the binding of the SH2 domains with dually phosphorylated ITAM domains on transmembrane proteins disrupts these intramolecular interactions and allows the kinase domain to adopt an active conformation. Initial ITAM binding triggers rapid activation of Syk, whereas subsequent phosphorylation leads to prolonged activation. The phosphorylation of Syk and of the ITAM domains is essentially mediated by SRC subfamily kinases, like LYN, upon transmembrane receptors engagement. Syk can phosphorylate ITAMs and autophosphorylate its own interdomain region tyrosines, providing a positive feedback loop following initial ITAM-mediated Syk activation. Syk is also reported to mediate signaling of receptors that do not contain an ITAM, including integrins and C-type lectins, via interaction with ITAM-containing adaptors, such as DAP12 (TYROBP) and the FcR -chain (FcR ). This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mocsai, A., et al. (2010). Nat. Rev. Immunol. 10(6); 387-402).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable