ZRB1338

Anti-MCU Antibody, clone 3N3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Calcium uniporter protein mitochondrial;HsMCU;Coiled-coil domain-containing protein 109A
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3N3, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 39.87 kDa; observed mol wt ~41 kDa
• 純化經由: using Protein A
• 物種活性: mouse, human
• 物種活性（以同源性預測）: bovine, canine, rat, feline, monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_612366
• UniProt登錄號: Q8NE86
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 3N3 is a Rabbit recombinant monoclonal antibody that specifically detects Calcium uniporter protein, mitochondrial (MCU). It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Calcium uniporter protein, mitochondrial (MCU).

應用

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MCU in A549 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MCU in PANC-1 and NIH3T3 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound MCU with a KD of 2.0 x 10-6 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MCU in A549 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Calcium uniporter protein, mitochondrial (UniProt: Q8NE86; also known as HsMCU, Coiled-coil domain-containing protein 109A, MCU) is encoded by the MCU (also known as C10orf42, CCDC109A) gene (Gene ID: 90550) in human. MCU is a multi-pass inner mitochondrial membrane calcium uniporter that mediates calcium uptake into mitochondria. It is synthesized with a transit peptide (aa 1-50), which is subsequently cleaved off to generate the mature MCU protein that has two mitochondrial matrix domains (aa 51-233 and 284-351), two transmembrane domains (aa 234-256 and 266-283) and one mitochondrial Intermembrane domain (aa 257-265). Its activity is regulated by the mitochondrial calcium uptake 1 (MICU1) and MICU2. MICU1 is a single pass membrane protein that helps to stabilize the entire mitochondrial calcium uniporter complex. It also limits the amount of calcium that enters the cell during low concentrations of calcium. Also, it functions alongside MCU to keep the accumulated calcium inside the mitochondrial matrix. MICU2 works with MICU1 and MCU to reduce the amount of calcium coming into the matrix. At low calcium levels MCU activity is down-regulated by MICU1 and MICU2 and at higher calcium levels MICU1 increases MCU activity. Synaptic activity is also reported to transcriptionally represses MCU gene, via a mechanism involving the nuclear Ca2+ and CaM kinase-mediated induction of Npas4, resulting in the inhibition of NMDA receptor-induced mitochondrial Ca2+ uptake and preventing excitotoxic death. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Marchi, S., and Pinton, P. (2014). J. Physiol. 592(5); 829-839; Qiu, J., et al. (2013). Nat. commun. 4; Article 2034; De Stefani, D., et al. (2011). Nature 476 (7360); 336-340).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 13 - Non Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable