ZRB1332

Anti-DAXX Antibody, clone 1L6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1L6, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 81.37 kDa; observed mol wt ~408 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_001135441
• UniProt登錄號: Q9UER7
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1L6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Death domain-associated protein 6 (DAXX). It targets an epitope within the C-terminal half.

免疫原

His-tagged recombinant fragment corresponding to 269 amino acids from the C-terminal half of human Death domain-associated protein 6 (DAXX).

應用

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected DAXX in Ramos cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected DAXX in HeLa and Raji cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound recombinant DAXX protein fragment with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DAXX in human testis tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected DAXX in HeLa cell line, A431 and HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-DAXX, clone 1L6 ZooMAb^®, Cat. No. ZRB1332, is a recombinant Rabbit monoclonal antibody that specifically targets DAXX and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

標靶描述

Death domain-associated protein 6 (UniProt: Q9UER7; also known as Daxx, hDaxx, ETS1-associated protein 1, EAP1, Fas death domain-associated protein, DAXX) is encoded by the DAXX (also known as BING2, DAP6) gene (Gene ID: 1616) in human. DAXX is a homomultimeric protein that is dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli. It serves as a transcription corepressor that represses transcriptional potential of several sumoylated transcription factors. It down-regulates basal and activated transcription and its repressor activity is modulated by its recruitment to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. It is reported to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may thereby influence TNFRSF6-dependent apoptosis. It also acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress conditions, in association with the deubiquitinating USP7, it prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53 that leads to TP53 ubiquitination and proteasomal degradation. However, upon DNA damage, its association with MDM2 and USP7 is disrupted, which results in increased MDM2 autoubiquitination and consequently, MDM2 degradation and TP53 stabilization. It also acts as targeting component of the chromatin remodeling complex ATRX:DAXX that has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats. However, it does not affect the ATPase activity of ATRX, but alleviates its transcription repression activity. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1