生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1E13, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt ~112.90 kDa
observed mol wt ~100 kDa
物種活性
human
物種活性（以同源性預測）
bovine, rat, monkey, mouse
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
, Aligned
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1E13 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Na(+)/K(+) ATPase a1 subunit. It targets an epitope within 16 amino acids from the third cytoplasmic domain from the N-terminal half.
免疫原
KLH-conjugated linear peptide corresponding to 16 amino acids from the third cytoplasmic domain from the N-terminal half of human Na(+)/K(+) ATPase a1 subunit.
應用
Quality Control Testing
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Na+-K+ ATPase a1 in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Na+-K+ ATPase 1 in MCF-7, A549. and Human colon lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Na+-K+ ATPase 1 in A549 cell line.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Na+-K+ ATPase 1 in human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Na+-K+ ATPase a1 in A431 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Na+-K+ ATPase 1 in MCF-7, A549. and Human colon lysates.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Na+-K+ ATPase 1 in A549 cell line.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Na+-K+ ATPase 1 in human colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Na+-K+ ATPase a1, clone 1E13 ZooMAb®, Cat. No. ZRB1266, is a rabbit recombinant monoclonal antibody that specifically targets of Na+-K+ ATPase a1 and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
標靶描述
Sodium/potassium-transporting ATPase subunit alpha-1 (UniProt: P05023; also known as EC:7.2.2.13, Na (+)/K (+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1) is encoded by the ATP1A1 gene (Gene ID: 476) in human. Na (+)/K (+) ATPase alpha-1 is a multi-pass membrane protein with five extracellular domains; ten helical domains, and six cytoplasmic domains. It is synthesized with a short propeptide (aa 1-5), which is removed in the mature form. It serves as the catalytic component of the active enzyme that catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions that provides energy for active transport of various nutrients. This gradient is also essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. Phosphorylation of Na (+)/K (+) ATPase alpha-1 on tyrosine 10 modulates its pumping activity and phosphorylation on serine 943 by protein kinase A modulates its response protein kinase C. Following increase in intracellular sodium, it undergoes dephosphorylation by protein phosphatase 2A (PP2A) that leads to increase in its catalytic activity. Mutations in ATP1A1 gene have been linked to Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
外觀
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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