ZRB1107

Anti-Recoverin Antibody, clone 1J23, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1J23, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 23.13 kDa; observed mol wt ~21 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable (peptide)
• 同型: IgG
• UniProt登錄號: P35243
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RCVRN(5957)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1J23 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human Recoverin.

免疫原

His-tagged recombinant full-length human Recoverin.

應用

Anti-Recoverin, clone 1J23 ZooMAb , Cat. No. ZRB1107, is a recombinant rabbit monoclonal antibody that specifically targets Recoverin and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Recoverin in ARPE-19 cells treated with 3 uM fenretinide for 7 days.

Immunofluorescence Analysis: A 1:1,250 dilution from a representative lot detected Recoverin in human retina tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,250 dilution from a representative lot detected Recoverin in human retina tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Recoverin (UniProt: P35243; also known as Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known s RCV1) gene (Gene ID: 5957) in human. Recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins that is expressed in retinal photoreceptors and midget cone bipolar cells. It unctions as a calcium sensor that regulates rhodopsin phosphorylation through inhibition of rhodopsin kinase. It contains four EF hand motifs (aa 25-60; 61-96; 97-132; 147-182) and a myristoyl chain on the N-terminus. It has a high affinity (aa 110-121) and a low affinity (aa 74-85) calcium binding site. Recoverin undergoes a conformational change upon calcium binding in darkness and subsequently it attaches to the disc membrane in photoreceptor outer segment. This in turn allows binding of G protein-coupled receptor kinases (GRK). Calcium-bound Recoverin is shown to inhibit G-protein-coupled receptor kinase (GRKs) in darkness, which prevents visual pigment phosphorylation. Under bright light condition, calcium dissociates from Recoverin and the protein translocates to the inner segment. The released GRK can phosphorylate and deactivate the light activated visual pigment. Mutations in Recoverin have been linked to retinal dysfunction associated with early-stage diabetes and cancer-associated retinopathy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zang. J., and Neuhauss, SCF (2018). Front. Mol. Neurosci 11:473).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable