ZRB1048

Anti-DOCK11 Antibody, clone 1F11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: ACG, Activated Cdc42-associated guanine nucleotide exchange factor, Dedicator of cytokinesis protein 11, Zizimin-2
• 分類程式碼代碼: 12352203

屬性

• 生物源: rabbit (recombinant)
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1F11, recombinant monoclonal; monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 237.67 kDa; observed mol wt 238 kDa
• 物種活性: rat, mouse, human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: Q5JSL3
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... DOCK11(139818)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1F11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects DOCK11 protein. It targets an epitope within 25 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal half of human DOCK11 protein.

應用

Anti-DOCK11, clone 1F11 ZooMAb, Cat. No. ZRB1048, is a recombinant Rabbit monoclonal antibody that specifically targets DOCK11 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DOCK11 in HEK293 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected DOCK11 in HeLa, A431, HepG2, and NIH3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound DOCK11 peptide with a KD of 5.7 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Dedicator of cytokinesis protein 11 (UniProt: Q5JSL3; also known as Activated Cdc42-associated guanine nucleotide exchange factor, ACG, Zizimin-2, DOCK11) is encoded by the DOCK11 (also known as ZIZ2) gene (Gene ID: 139818) in human. DOCK11 is a guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. It is shown to be essential for marginal zone (MZ) B-cell development and associated with early bone marrow B-cell development, MZ B-cell formation, MZ B-cell number and marginal metallophilic macrophages morphology. DOCK11 contains DHR-1 (aa 640-880) and DHR-2 (aa 1609-2036) domains. The DHR-2 domain is essential for its GEF activity and mediates interaction with activated CDC42 in conjunction with residues 66-126. DOCK11 expression has been reported in Germinal center B lymphocytes, spleen, thymus, bone marrow, and in peripheral blood lymphocytes. Lower levels of expression have been observed in NIH-3T3 fibroblasts, 2C12 myoblasts and Neuro-2A neuroblastoma cells.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1