ZRB1031

Anti-Siglec-9 Antibody, clone 3F22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 3F22, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 50.08 kDa; observed mol wt ~670 kDa
• 物種活性: mouse, human
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: flow cytometry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_055256.1
• UniProt登錄號: Q9Y336
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 3F22 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Siglec-9. It targets an epitope within 15 amino acids from the C-terminal, cytoplasmic domain.

免疫原

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal, cytoplasmic domain of human Siglec-9.

應用

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Siglec-9 in Jurkat cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Siglec-9 in HepG2 and Raw264.7 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Siglec-9 in human spleen tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected Siglec-9 in one million Human peripheral blood monouclear cells (PBMC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Siglec-9, clone 3F22 ZooMAb^®, Cat. No. ZRB1031, is a recombinant Rabbit monoclonal antibody that detects Siglec-9 and is tested for se in Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

標靶描述

Sialic acid-binding Ig-like lectin 9 (UniProt: Q9Y336; also known as Siglec-9, CDw329, Protein FOAP-9, CD329) is encoded by the SIGLEC9 (also known as UNQ668/PRO1302) gene (Gene ID: 27180) in human. Sialic-acid-binding immunoglobulin-like lectins (Siglecs) belong to a lectin family of surface receptors that are predominantly expressed on hematopoietic cells with cell type and differentiation specificity. By binding with sialic acids at the terminal position of glycoproteins and lipids they regulate the intensity and duration of innate or adaptive immune response. Siglec-9 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 18-348), a transmembrane domain (aa 349-369), and a cytoplasmic domain (aa 370-463). It has one Ig-like V-type domain (aa 20-140) and two Ig-like C2-type domains (aa 146-229 and 236-336). It also contains one ITIM motif (aa 431-436) that is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-continaing phosphatases. It is expressed by peripheral blood leukocytes and is also found in a variety of other tissues at lower levels. Siglec9 serves as a putative adhesion molecule that mediate sialic-acid dependent binding to cells. It preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Reduced expression of Siglec-9 on natural killer cells has been linked to persistent hepatitis B virus replication. Increased expression of soluble Siglec-9 has been reported in subjects with chronic obstructive pulmonary disease. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhao, D., et al. (2018). Front. Immunol. 9: 1124; Zeng, Z., et al. (2017). Sci. Rep. 7; Article 10116).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable