ZMS1042

Anti-ssDNA Antibody, clone 16-19 ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Single stranded DNA
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 16-19, recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 純化經由: using protein G
• 物種活性: bovine
• 物種活性（以同源性預測）: all
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 16-19 is a ZooMAb^® Mouse recombinant monoclonal antibody that specifically detects ssDNA.

免疫原

Hybridoma generated by fusion of splenocytes from non-immunized BW and myeloma cell line Sp 2/0-Ag 14.

應用

Quality Control Testing

Evaluated by Dot Blot with ssDNA from Calf thymus.

Dot Blot Analysis: A 1:1,000 dilution of this antibody detected ssDNA from Calf thymus.

Tested applications

Enzyme Immunoassay Analysis (ELISA): Serial of dilutions from a representative lot detected ssDNA from Calf thymus.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ssDNA in Molt 4 cells treated with Etoposide (10 M; overnight).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Dot Blot with ssDNA from Calf thymus.

Dot Blot Analysis: A 1:1,000 dilution of this antibody detected ssDNA from Calf thymus.

標靶描述

DNA, the carrier of genetic information, is a crucial part of basic biological research and synthetic biology. It exists as a double-helix structure and the two DNA strands known as polypeptides are composed of monomeric units known as nucleotides. Each nucleotide consists of four bases: adenine, guanine, cytosine, thymine and a deoxyribose sugar moiety. The nitrogenous bases of the two separate strands are bound together by base pairing (A-T and C-G) to create a double stranded DNA. Under certain conditions, these two strands can come apart (melting) to generate two single-stranded DNA. Single-stranded DNA (ssDNA) is a crucial DNA damage signal found at the sites of DNA damage that helps in recruitment of checkpoint kinases to the site of damage. This leads to cell cycle arrest, transcriptional upregulation of DNA repair genes, and modulation of DNA replication pathways. Hence, ssDNA is considered as a key intermediate in nearly all biochemical reactions involving DNA replication and DNA repair. However, the ssDNA structure is not stable and single-stranded DNA-binding proteins (SSB) hold the ssDNA intact and exposed during the course of its function, such as DNA transcription, recombination, and repair, and serve as template for opposite strand DNA synthesis. Antibodies against ssDNA are commonly used in ELISA-based test for the quantitative determination of IgG antibodies against ssDNA and have been used in serological detection of systemic lupus erythematosus (SLE) and drug induced lupus. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rekvig, OP. (2022). Front. Immunol. 12; 808008; Bantele, SCS., et al. (2019). Nat. Commun. 10; Article 944; Ballard, DW., and Voss EW. (1985). J. Immunol. 135(5); 3372-3380).

外觀

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable