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Merck
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重要文件

Z0377

Sigma-Aldrich

Anti-Zyxin antibody, Mouse monoclonal

clone ZOL301, purified from hybridoma cell culture

同義詞:

Anti-ESP-2, Anti-HED-2

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

ZOL301, monoclonal

形狀

buffered aqueous solution

分子量

antigen 82-84 kDa

物種活性

mouse, rat, human

包裝

antibody small pack of 25 μL

技術

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of HeLa cells.

同型

IgG2a

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... ZYX(7791)
mouse ... Zyx(22793)
rat ... Zyx(114636)

一般說明

Anti-Zyxin antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the hybridoma ZOL301 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human Zyxin conjugated to KLH via an N-terminal added cysteine. Zyxin is a relatively low abundance component of focal adhesion plaques, stress fibers, and dynamic cell membrane areas such as the leading edge. It may also be found in the cell nucleus. Zyxin is a member of the LIM domain protein family that also includes LPP (Lipoma preferred partner), Trip6 (thyroid hormone interacting protein 6), and Ajuba. It is a phosphoprotein and comprises two major domains, an extended N-terminal domain that contains proline-rich sequence, and a C-terminal domain that contains a functional nuclear export signal and three double zinc-finger cysteine and histidine-rich LIM domains.

應用

Anti-Zyxin antibody, Mouse has been used in:
  • Western blotting
  • Immunofluorescence staining
  • Enzyme linked immunosorbent assay (ELISA)
  • Immunocytochemistry

生化/生理作用

Zyxin interacts with multiple cytoskeletal, signaling and other proteins such as α -actinin, vasodilator-stimulated phosphoprotein (VASP), Mena, LIM-only cysteine-rich proteins (CRP1, CRP2, CRP3/MLP), Vav, breast cancer anti-estrogen resistance protein 1 (p130Cas), CRK-associated substrate-related protein/ (Human Enhancer of Filamentation 1) (CasL/HEF1, and serine-arginine(SR)-related protein (SON) DNA-binding protein. During mitosis a fraction of zyxin was reported to associate with the tumor suppressor h-warts/LATS1 (large tumor suppressor kinase 1) on the mitotic apparatus. Zyxin has been implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. Involvement of zyxin in gene transcription by its association with transcription factors in the nucleus has been suggested.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling
Hoffman LM, et al.
The Journal of Cell Biology, 172(5), 771-782 (2006)
Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor
Hirota T, et al.
The Journal of Cell Biology, 149(5), 1073-1086 (2000)
Zyxin and paxillin proteins: focal adhesion plaque LIM domain proteins go nuclear
Wang Y and Gilmore TD
Biochimica et Biophysica Acta - Molecular Cell Research, 1593(2-3), 115-120 (2003)
Identification of intracellular proteins and signaling pathways in human endothelial cells regulated by angiotensin-(1-7)
Meinert C, et al.
Journal of Proteomics, 130, 129-139 (2016)
Alexandra Woychek et al.
Cytoskeleton (Hoboken, N.J.), 76(2), 200-208 (2019-01-23)
The nuclear envelope protein nesprin-2G is a component of the linker of nucleoskeleton and cytoskeleton (LINC) complex and is responsible for mechanical and signaling crosstalk between the nucleus and cytoskeleton. A prior study has demonstrated that nesprin-2G knockout mice show

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