Z0377

Anti-Zyxin antibody, Mouse monoclonal

clone ZOL301, purified from hybridoma cell culture

• 同義詞: Anti-ESP-2, Anti-HED-2
• MDL號碼: MFCD01634364
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: ZOL301, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 82-84 kDa
• 物種活性: mouse, rat, human
• 包裝: antibody small pack of 25 μL
• 技術: immunocytochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of HeLa cells.
• 同型: IgG2a
• UniProt登錄號: Q15942
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZYX(7791) ; mouse ... Zyx(22793) ; rat ... Zyx(114636)

描述

一般說明

Anti-Zyxin antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the hybridoma ZOL301 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to human Zyxin conjugated to KLH via an N-terminal added cysteine. Zyxin is a relatively low abundance component of focal adhesion plaques, stress fibers, and dynamic cell membrane areas such as the leading edge. It may also be found in the cell nucleus. Zyxin is a member of the LIM domain protein family that also includes LPP (Lipoma preferred partner), Trip6 (thyroid hormone interacting protein 6), and Ajuba. It is a phosphoprotein and comprises two major domains, an extended N-terminal domain that contains proline-rich sequence, and a C-terminal domain that contains a functional nuclear export signal and three double zinc-finger cysteine and histidine-rich LIM domains.

應用

Anti-Zyxin antibody, Mouse has been used in:

• Western blotting
• Immunofluorescence staining
• Enzyme linked immunosorbent assay (ELISA)
• Immunocytochemistry

生化／生理作用

Zyxin interacts with multiple cytoskeletal, signaling and other proteins such as α -actinin, vasodilator-stimulated phosphoprotein (VASP), Mena, LIM-only cysteine-rich proteins (CRP1, CRP2, CRP3/MLP), Vav, breast cancer anti-estrogen resistance protein 1 (p130Cas), CRK-associated substrate-related protein/ (Human Enhancer of Filamentation 1) (CasL/HEF1, and serine-arginine(SR)-related protein (SON) DNA-binding protein. During mitosis a fraction of zyxin was reported to associate with the tumor suppressor h-warts/LATS1 (large tumor suppressor kinase 1) on the mitotic apparatus. Zyxin has been implicated in the regulation and modulation of actin filaments organization, cell adhesion, cell motility, mitosis, and signal transduction. Zyxin may shuttle between focal adhesion plaques and the nucleus. Involvement of zyxin in gene transcription by its association with transcription factors in the nucleus has been suggested.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)