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重要文件

Y2627

Sigma-Aldrich

2xYT Medium

EZMix Powder microbial growth medium

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

等級

for molecular biology

描述

nutrient rich, quick dissolve, dust free formulation

無菌

non-sterile

形狀

powder

成份

Inert binder (EZMix only), 0.6 g/L
NaCl, 5 g/L
Tryptone (pancreatic digest of casein), 16 g/L
Yeast extract, 10 g/L

應用

agriculture
food and beverages

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

一般說明

2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

應用

Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

特點和優勢

2xYT EZMix powder provides:
  • Granulated, dust-free format for safer handling and faster mixing
  • Easy scale-up using larger package size
  • Standard formulation

重構

Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

法律資訊

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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條款

General protocols for growth of competent cells in microbial medium.

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