X0879

Anti-XPB 兔抗

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-BTF2, Anti-ERCC3, Anti-Excision repair cross-complementing rodent repair deficiency, complementation group 3
• MDL號碼: MFCD02263308
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 90 kDa
• 物種活性: human, rat, mouse
• 濃度: ~1 mg/mL
• 技術: indirect immunofluorescence: 10-20 μg/mL using HeLa cells fixed with paraformaldehyde-Triton; western blot: 1-2 μg/mL using HepG2 cell lysates
• UniProt登錄號: P19447
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ERCC3(2071) ; mouse ... Ercc3(13872) ; rat ... Ercc3(291703)

描述

一般說明

XPB (Xeroderma pigmentosum complementation group B) is located on the human chromosome at 2q14.3. TFIIH (General transcription and DNA repair factor IIH helicase) consists of nine protein subunits, XPB (ERCC3) being one of them. It is an ATP-dependent DNA helicase of 89 kDa. The XPB is localized in the neurons.

免疫原

synthetic peptide corresponding to amino acids 765-782 of human XPB, also known as ERCC3, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the mouse and rat sequences in one and three amino acids respectively.

應用

Anti-XPB antibody has been used in immunoblotting andimmunofluorescence.

生化／生理作用

XPB (General transcription and DNA repair factor IIH helicase subunit XPB) is involved in DNA repair and transcription. Phosphorylation on Ser⁷⁵¹ of XPB inhibits TFIIH repair activity, leaving its transcription function intact. Mutations in the gene encoding XPB causes Xeroderma Pigmentosum, Cockayne′s syndrome, and trichothiodystrophy.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)