推薦產品
描述
125 μL maximum volume/sample area, 500 μL maximum total volume/card
包裝
pkg of 100 ea
製造商／商標名
Qiagen WB120065
一般說明
The 4 spot area QIAcard™ FTA™ PlantSaver format provides a laminated flap that allows you to vigorously crush plant or insect samples into the FTA™ matrix without damaging the FTA™ card.
Applications:
Analyze plant DNA by PCR-based assays, Detection of Genetically Modified (GM) organisms, Marker selected breeding, Varietal identification, Phylogeny analysis, Amplification of low copy number loci, Invader assays, Multiple displacement amplification, Transgenic identification.
Analysis:
Plant tissue is physically crushed on the card and the DNA binds to the matrix.
The chemical coating on the FTA™ card protects the DNA from degradation and allows the cards to be stored at room temperature for extended periods of time. To prepare the sample for PCR, the matrix is washed with two nontoxic aqueous buffers.
The DNA remains bound to the matrix throughout purification and a small disk (1.2 or 2 mm in diameter) of the matrix provides enough template for PCR analysis.
Applications:
Analyze plant DNA by PCR-based assays, Detection of Genetically Modified (GM) organisms, Marker selected breeding, Varietal identification, Phylogeny analysis, Amplification of low copy number loci, Invader assays, Multiple displacement amplification, Transgenic identification.
Analysis:
Plant tissue is physically crushed on the card and the DNA binds to the matrix.
The chemical coating on the FTA™ card protects the DNA from degradation and allows the cards to be stored at room temperature for extended periods of time. To prepare the sample for PCR, the matrix is washed with two nontoxic aqueous buffers.
The DNA remains bound to the matrix throughout purification and a small disk (1.2 or 2 mm in diameter) of the matrix provides enough template for PCR analysis.
特點和優勢
- Simply pressing plant samples onto the FTA™ matrix or applying homogenate captures and stabilizes the DNA.
- Store and collect at room and high temperatures for convenient collection in the field.
- Smaller sample size: Only young leaves are required, reducing growing time and speeding research.
- Fast purification in 30 minutes: PCR-ready DNA can be purified on FTA™ in three simple steps, all in a single tube at room temperature.
其他說明
Sample integrity is optimized when FTA™ Cards are stored in a Multi-Barrier Pouch with a Desiccant Packet.
Field of Use : For internal research use only
Field of Use : For internal research use only
法律資訊
FTA is a trademark of Qiagen Group
QIAcard is a trademark of Qiagen Group
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文章
This article describes the evaluation of Whatman FTA cards from GE Healthcare for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
條款
This study examined the yield and quality of DNA from samples applied to Whatman FTA cards, using five common methods of DNA extraction.
This page shows options for sample collection, transport, archiving, and DNA purification.
Nucleic Acid Sample Preparation Methodology
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