V4889

Anti-phospho-Vinculin (pTyr⁸²²) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-CMD1W, Anti-CMH15, Anti-HEL114, Anti-MV, Anti-MVCL
• MDL號碼: MFCD06798505
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 物種活性: human, chicken, rat, mouse
• 技術: western blot: 1:1,000 using chick embryo fibroblasts (CEFs) transfected with activated Src.
• UniProt登錄號: P18206
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pTyr822)
• 基因資訊: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

描述

一般說明

Vinculin (VCL) is a ubiquitously expressed cytoskeletal protein, encoded by the gene mapped to human chromosome 10q11.2-qter. VCL is a vital element of adherens type cell junctions. Vinculin structure contains a globular head connected to a tail domain by a short proline-rich sequence, and an intramolecular interaction between the head and tail hides the various ligand-binding sites in the protein.

免疫原

synthetic phosphopeptide derived from the region of vinculin that contains tyrosine 822.

應用

Anti-phospho-Vinculin (pTyr822) antibody produced in rabbit has been used in immunofluorescence staining.

生化／生理作用

Vinculin (VCL) is a cytoskeletal protein that supports cell-to-cell and cell to matrix junctions. It is also essentially involved in normal embryonic development and cardiac function, due to its role in controlling adhesion and motility, as well as cell spreading.In mature focal adhesions (FAs), VCL acts as an important constituent of the “molecular clutch” that induces the transmission of force from cytoplasmic F-actin to membrane-bound integrins. Aberration or mutation of VCL leads to cardiomyopathy, which is associated with hypertension. Experimental studies reveal that, VCL might be used as a biomarker of aging or heart failure. Vinculin associates with FAs and assists in several cellular functions, such as stabilizing and strengthening FAs and promoting their maturation, extracellular matrix (ECM) mechanosensing, regulating actin cytoskeletal dynamics and signaling pathways involved in apoptosis.

外觀

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)