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TRUEDEXF

Sigma-Aldrich

TrueGel3D聚合物

Modified for fast gelling (FAST-DEXTRAN),allow cell recovery

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

運輸包裝

dry ice

儲存溫度

−70°C

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一般說明

Dextrose polymers are functionalized with thiol-reactive maleimide groups, which react rapidly with thiol groups of crosslinkers to form hydrogels in few seconds. The thiol-reactive maleimide groups are also compatible to conjugate polymers with bioactive factors. The thioglycerol is used to maintain hydrogel strength and also as a control to compare cellular behavior in peptide-modified versus unmodified hydrogels. The buffers (pH7.2 and pH 5.5) are used to control pH and osmotic conditions to optimize time for gelation.

TrueGel3D enzymatic cell recovery solution (TRUEENZ-500UL) is used to dissolve dextran polymers for easy recovery of chemically fixed or live cells for post-culture analyses including RT-PCR, western blots and other molecular biological studies.

應用

FAST-DEXTRAN polymer is used with crosslinkers (CD/PEG crosslinkers) to establish stable hydrogels

特點和優勢

  • Contains dextrose polymer functionalized with maleimide groups for quick gelation
  • Degradable polymer

成分

TRUEDEXF contains FAST-DEXTRAN lyophilized with reconstitution buffer, TrueGel3D thioglycerol and water

注意

Avoid frequent freeze-thaw cycles and store FAST-DEXTRAN in small portions

象形圖

Corrosion

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials


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文章

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

相關內容

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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