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TDF100A

Supelco

总膳食纤维测定试剂盒

sufficient for ~100 assays

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About This Item

分類程式碼代碼:
12164500
NACRES:
NA.84

用途

sufficient for ~100 assays

品質等級

技術

photometry: suitable

應用

food and beverages
general analytical

儲存溫度

2-8°C

應用

此检测通过酶促和比重联合法来测定食物中的总膳食纤维含量。 先使用热稳定的α-淀粉酶对干燥的脱脂食物样品进行凝胶化,然后通过蛋白酶和淀粉葡糖苷酶进行酶促消化,以除去样品中存在的蛋白质和淀粉。 之后加入乙醇来沉淀可溶性的膳食纤维。 过滤剩余物质,并使用乙醇和丙酮进行洗涤。 干燥后对剩余物质进行称重。 一半样品进行蛋白质分析,其余进行灰化。 总膳食纤维为扣除蛋白质和灰的重量之后的残余物质重量。

其他說明

检测总膳食纤维的试剂。

原則

使用酶促和比重分析联合方法。 本操作步骤基于已发表的官方分析方法(Official Methods of Analysis),15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990) 。

僅套裝組件

產品號碼
描述

  • α-Amylase, Heat-stable 10 mL

  • Amyloglucosidase 10 mL

  • Celite 50 g

  • Protease 500 mg

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

標靶器官

Lungs, Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Apurba Giri et al.
Journal of food science and technology, 54(8), 2443-2451 (2017-07-26)
To develop a functional processed cheese spread (PCS) different levels of inulin (0, 4, 6 and 8%) addition into PCS was studied with its physico-chemical, sensory and fatty acid profile and micro-structural quality. As the level of inulin addition increased
Ashrafi Hossain et al.
Food chemistry, 199, 648-652 (2016-01-19)
This article decribes the nutrient composition of four strawberry genotypes cultivated at the Sher-e-Bangla Agriculture University horticulture farm in Dhaka (Bangladesh). AOAC and standard validated methods were employed to analyse the nutrient composition. Protein, fat and ash contents were found
Isabella Corsato Alvarenga et al.
PloS one, 13(10), e0206090-e0206090 (2018-10-27)
Sorghum is an abundant starch source that has many potential health benefits. Some pet food companies have adopted whole sorghum in their formulations, however sorghum flour and (or) its phenolic rich seed coat might provide added benefit to companion animal
Vanessa Gressler et al.
Journal of food protection, 81(8), 1258-1263 (2018-07-07)
A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In brief, the samples were
Ang-Xin Song et al.
International journal of biological macromolecules, 133, 957-963 (2019-04-28)
The efficacy of probiotic health products depends on the capability of the constituent probiotic bacteria to survive through long period of cold storage and the gastrointestinal tract. This study was to evaluate the protective effects of a high-molecular weight (MW)

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Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

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