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Key Documents

T8535

Sigma-Aldrich

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse

clone JDR.3B8, ascites fluid

同義詞:

Anti-CDCBM5, Anti-TUBB, Anti-TUBB2

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

JDR.3B8, monoclonal

包含

15 mM sodium azide

物種活性

bovine, pig, chicken, human, rat, mouse

包裝

antibody small pack of 25 μL

技術

indirect ELISA: suitable
indirect immunofluorescence: suitable
microarray: suitable
western blot: 1:1,000 using a chicken fibroblast extract or a chicken brain microtubule proteins preparation

同型

IgG2b

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with a core of two β-sheets enclosed by α-helices. In addition, it also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. β-tubulin I and II is encoded by the gene mapped to human chromosome 20 and 6p25.2 respectively.
Monoclonal Anti-β-Tubulin Isotype I + II (mouse IgG2b isotype) is derived from the JDR.3B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

特異性

The antibody specifically recognizes an epitope located on isotypes I and II of β-tubulin. No cross-reactivity with other tubulin isotypes is observed.

免疫原

synthetic peptide corresponding to the C-terminal sequence of human β-tubulin isotype II coupled to BSA

應用

Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in following studies:
  • immunoblot
  • immunofluorescence technique
  • immunohistochemistry.
Monoclonal Anti-β-Tubulin I+II antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • western blotting
  • immunoblotting
  • dot blot
  • immunocytochemistry

生化/生理作用

β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP). Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Tubulin β 2B class IIb (TUBB2B)/β-tubulin II plays a vital role in migration/organization and axon guidance processes. Mutation in β-tubulin gene is associated with the development of asymmetric polymicrogyria (PMG).

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

ABCA3 as a Lipid Transporter in Pulmonary Surfactant Biogenesis
Ban N
The Journal of Biological Chemistry, 282, 9628-9634 (2007)
Cytoskeleton in action: Lissencephaly, a neuronal migration disorder
Moon HM
Wiley interdisciplinary reviews. Developmental biology, 2, 229-245 (2013)
Jeffrey K Huang et al.
BMC developmental biology, 7, 59-59 (2007-06-02)
Trigeminal nerves consist of ophthalmic, maxillary, and mandibular branches that project to distinct regions of the facial epidermis. In Xenopus embryos, the mandibular branch of the trigeminal nerve extends toward and innervates the cement gland in the anterior facial epithelium.
Postnatal refinement of auditory hair cell planar polarity deficits occurs in the absence of Vangl2.
Copley CO
The Journal of Neuroscience, 33, 14001-14016 (2013)
A novel mutation in the β-tubulin gene TUBB2B associated with complex malformation of cortical development and deficits in axonal guidance.
Romaniello R
Developmental Medicine and Child Neurology, 54, 765-769 (2012)

文章

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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