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Key Documents

T8453

Sigma-Aldrich

Anti-β-Tubulin II antibody, Mouse monoclonal

clone 7B9, purified from hybridoma cell culture

同義詞:

Anti-TUBB2, Anti-Tubulin, β2

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

unconjugated

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

7B9, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~50 kDa

物種活性

human, mouse, rat

濃度

~1.0 mg/mL

技術

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

一般說明

Monoclonal Anti- β-Tubulin II (mouse IgG1 isotype) is derived from the hybridoma 7B9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment at the C-terminus of human β?tubulin II. Tubulin is the major building block of microtubules. This intracellular, cylindrical, filamentous structure is present in almost all eukaryotic cells. Tubulin is a heterodimer that consists of α-tubulin and β ?tubulin. Both subunits have a molecular mass of ~50 kDa and share considerable homology.

應用

Anti-β-Tubulin II antibody has been used in
  • immunoblotting immunoprecipitation
  • immunocytochemistry
  • western blotting
  • immunolabelling

生化/生理作用

The αβ tubulins are involved in the formation of microtubules during cell division by the formation of protofilaments.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Hongfeng Yuan et al.
Oncotarget, 8(9), 14912-14924 (2017-01-25)
Diffuse large B-cell lymphoma (DLBCL) is one of the most aggressive non-Hodgkin lymphomas. It is curable but one-third of cases are refractory to therapy or relapse after initial response highlighting the urgent need for developing novel therapeutic approaches. Targeting sirtuins
Mutations affecting beta-tubulin folding and degradation
Wang Y, et al.
The Journal of Biological Chemistry, 281(19), 13628-13635 (2006)
Spatio-temporal dynamics of $\beta$-tubulin isotypes during the development of the sensory auditory organ in rat
Renauld J, et al.
Histochemistry and Cell Biology, 144(5), 403-416 (2015)
cGMP inhibits TGF-beta signaling by sequestering Smad3 with cytosolic beta 2-tubulin in pulmonary artery smooth muscle cells
Gong K, et al.
Molecular Endocrinology, 25(10), 1794-1803 (2011)
Salama A Salama et al.
Fertility and sterility, 98(1), 178-184 (2012-05-15)
To investigate the effects and the mechanism of action of 2-methoxyestradiol (2ME(2)) on transforming growth factor (TGF) β3-induced profibrotic response in immortalized human uterine fibroid smooth muscle (huLM) cells. Laboratory study. University research laboratory. Not applicable. Not applicable. huLM cells

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