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T4444

3, 3′,5 ,5′-Tetramethylbenzidine

Name: 3, 3′,5 ,5′-Tetramethylbenzidine
Brand: SIGMA

peroxidase substrate, chromogenic, aqueous solution

同義詞:

TMB

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About This Item

CAS號碼:

54827-17-7

MDL號碼:

MFCD00007748

分類程式碼代碼:

12352204

PubChem物質ID:

24900209

NACRES:

NA.83

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product name

3,3′,5,5′-四甲基联苯胺液体底物，超灵敏，用于 ELISA, ready to use solution

品質等級

200

形狀

aqueous solution

運輸包裝

wet ice

儲存溫度

2-8°C

SMILES 字串

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI 密鑰

UAIUNKRWKOVEES-UHFFFAOYSA-N

一般說明

本品为包含3,3′,5,5′-四甲基联苯胺（TMB）的弱酸性缓冲液。底物以单组分即用型溶液提供。速率动力学约比传统TMB配方快40％。不推荐用于需要沉淀反应产物的膜或免疫组化应用。

應用

的3,3′,5,5′-四甲基联苯胺是超灵敏的ELISA液体底物，可用以下应用的作过氧化物酶底物：

通过转染子测定合成的透明质酸和HAS2（透明质酸合成酶）表达
测定结合的α-溶血素毒素的水平
结合IgG成像

3,3′,5,5′-四甲基联苯胺（TMB）是适用于ELISA程序的过氧化物酶底物。底物产生可溶的浅蓝色最终产物，并且可以在370或655nm下通过分光光度计读取。可以用2M H₂SO₄停止TMB反应（产生黄色溶液），并在450nm处读取数值。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

輸入產品批次/批號來搜索分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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Key Documents

3, 3′,5 ,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, aqueous solution

About This Item

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屬性

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品質等級

形狀

運輸包裝

儲存溫度

SMILES 字串

InChI

InChI 密鑰

相關類別

描述

一般說明

應用

安全資訊

儲存類別代碼

水污染物質分類（WGK）

閃點（°F）

閃點（°C）

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分析證明 (COA)

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