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Merck
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Key Documents

T1325

Sigma-Aldrich

Anti-Phosphotyrosine antibody produced in rabbit

0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

同義詞:

Anti-p-Tyr

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.44

生物源

rabbit

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

濃度

0.5 mg/mL

技術

indirect immunofluorescence: 3-6 μg/mL using A431 stimulated by human EGF.
western blot: 0.3-0.6 μg/mL using total cell extract of A431 stimulated by human EGF

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

phosphorylation (pTyr)

特異性

Anti-Phosphotyrosine recognizes tyrosine phosphorylated proteins.

免疫原

phospho-L-tyrosine conjugated to KLH

應用

Anti-Phosphotyrosine antibody is suitable for use in indirect immunofluorescence (3-6 μg/mL using A431 stiμLated by human EGF) and immunoblot (using U2OS Dr1 cells ). The antibody may also be used for immunoprecipitation (1:40 using rat lumbar and thoracic lysates) and immunocytochemistry.

生化/生理作用

Protein phosphorylation is the most abundant among post-translational modifications of cellular proteins, regulating intracellular signal transduction pathways in every living cell. Tyrosine, serine and threonine are the major amino acids that are phosphorylated in proteins. Antibodies specific for phosphotyrosine are essential tools for the characterization of tyrosine phosphorylation in many signal transduction pathways.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 1% BSA, containing 15 mM sodium azide.

儲存和穩定性

For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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