SRP7603

DNA Binding Protein-7 (DBP-7), recombinant

recombinant, expressed in E. coli, ≥99% (SDS-PAGE)

• 同義詞: DNA Binding Protein 7
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 重組細胞: expressed in E. coli
• 化驗: ≥99% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: ~9.4 kDa
• 包裝: pkg of 20 μg
• 雜質: endotoxin, tested
• NCBI登錄號: P39476
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... sso7d(1454006)

描述

一般說明

DNA Binding Protein-7 (DBP-7) is a N-terminal His-tagged recombinant protein and a member of the Sso7d family of small, abundant, non-specific DNA-binding proteins from the hyperthermophilic Archea Sulfolobus. The 7-kDa protein from Sulfolobus spp. consists of a five stranded, incomplete β-barrel capped at the opening by a C-terminal α-helix; they bind to the minor groove of a DNA duplex via the triple-stranded β-sheet. The topology of the Sulfolobus 7-kDa proteins was found to be similar to that of chromatin organization modifier (chromo) domains and eukaryotic SH3 domains, which are involved in protein–protein interactions. In vitro studies have shown that DBP-7 promotes the annealing of complementary DNA strands, induces negative supercoiling and chaperones the disassembly and renaturation of protein aggregates in an ATP hydrolysis-dependent manner.

外觀

Sterile filtered and Lyophilized without additives.

重構

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable