SRP4872

GDF9 人

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• 同義詞: GDF9, Growth/differentiation factor 9
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: ~31.2 kDa
• 包裝: pkg of 20 μg
• 雜質: endotoxin, tested
• NCBI登錄號: O60383
• UniProt登錄號: O60383
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... GDF9(2661)

描述

一般說明

GDF-9 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-3. Unlike most TGF-family members, GDF-9 and GDF-3 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. GDF-9 is expressed in oocytes and is required for normal ovarian folliculogenesis. Recombinant human GDF-9 is a 31.0 kDa non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains with 6XHis on its N-terminus.

應用

GDF9 has been as a supplement in the basal medium for the maturation of cumulus–oocyte complexes (COCs).

生化／生理作用

Growth differentiation factor 9 (GDF9) is known to be localized in the cytoplasm. The gene encoding it is localized on human chromosome 5q31.1. It is known to be associated with granulosa cell proliferation and steroid production. Downregulation of the gene is observed in polycystic ovary syndrome.

外觀

Sterile filtered and lyophilized with no additives.

重構

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable