SRP3239

GDNF from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 同義詞: ATF-1, Glial-Derived Neurotrophic Factor
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: rat
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: 15 kDa
• 包裝: pkg of 10 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: Q07731
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: rat ... GDNF(25453)

描述

一般說明

GDNF (glial cell derived neurotrophic factor) is a member of the transforming growth factor-β (TGF) superfamily. It is a potent neurotrophic factor which functions as a ligand for the multicomponent receptor complex containing GFRα1 (GDNF family receptor α1) and Ret receptor tyrosine kinase or neuronal cell adhesion molecule (NCAM). Rat GDNF is a homodimer linked by a disulfide bridge, and contains two glycosylated polypeptide chains that are released from the 211-residue-long precursor following a proteolytic cleavage.
The functional rat GDNF ligand is a disulfide-linked homodimer, of two 15.0kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

生化／生理作用

GDNF (glial cell derived neurotrophic factor) enhances motoneuron survival, and facilitates the survival of midbrain dopaminergic neurons which are damaged in degenerative diseases, such as Parkinson′s. In vivo and in vitro, the interaction of this protein with GDNF family receptor α1 and its co-receptor Ret tyrosine kinase, results in elevated neurite extension and survival of motor and sensory neurons. In rats with neuropathic pain, the injection of this protein in the locus coeruleus (LC) produces prolonged analgesia by increasing descending noradrenergic inhibition.

序列

MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI

外觀

Lyophilized from 10 mM Sodium Citrate.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable