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重要文件

SRP3238

Sigma-Aldrich

EGF 来源于大鼠

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

同義詞:

表皮生长因子

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32

暫時無法取得訂價和供貨情況

生物源

rat

重組細胞

expressed in E. coli

化驗

≥98% (HPLC)
≥98% (SDS-PAGE)

形狀

lyophilized

效力

≤0.1 ng/mL

分子量

6.2 kDa

包裝

pkg of 100 μg

技術

cell culture | mammalian: suitable

雜質

<0.1 EU/μg endotoxin, tested

顏色

white to off-white

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本產品
SML3206SML0184PHL89743
biological source

Panax notoginseng (Burk.) F.H.Chen

biological source

-

biological source

-

biological source

-

form

powder

form

powder

form

powder

form

solid

application(s)

metabolomics
vitamins, nutraceuticals, and natural products

application(s)

-

application(s)

-

application(s)

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

一般說明

Epidermal growth factor (EGF) is a potent growth factor and a cytoprotective peptide.[1] Recombinant rat EGF is a 6.2kDa globular protein containing 54 amino acid residues, including 3 intramolecular disulfide-bonds.

應用

Epidermal growth factor (EGF) is used in hepatocyte culture to determine the optimal condition for survival and function of rat hepatocytes.

生化/生理作用

Epidermal growth factor (EGF) stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, inflammation and is involved in wound healing.[2][3][1] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[4] This receptor also binds with TGF-α (transforming growth factor-α) and VGF (vaccinia virus growth factor).

序列

MNSNTGCPPS YDGYCLNGGV CMYVESVDRY VCNCVIGYIG ERCQHRDLRW WKLR

外觀

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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    The role of epidermal growth factor in prevention of oxidative injury and apoptosis induced by intestinal ischemia/reperfusion in rats.
    Arda-Pirinci P
    Acta Histochemica, 116(1), 167-175 (2014)
    Epidermal growth factor improves survival and prevents intestinal injury in a murine model of pseudomonas aeruginosa pneumonia.
    Dominguez JA
    Shock, 36(4), 381-389 (2011)
    The epidermal growth factor receptor family: biology driving targeted therapeutics.
    Wieduwilt MJ and Moasser MM
    Cellular and Molecular Life Sciences, 65(10), 1566-1584 (2008)
    Florence E Perrin et al.
    PloS one, 5(12), e15914-e15914 (2011-01-07)
    Spinal cord injury (SCI) is a widely spread pathology with currently no effective treatment for any symptom. Regenerative medicine through cell transplantation is a very attractive strategy and may be used in different non-exclusive ways to promote functional recovery. We
    Epidermal growth factor.
    G Carpenter et al.
    The Journal of biological chemistry, 265(14), 7709-7712 (1990-05-15)

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