SRP3140

PECAM-I human

recombinant, expressed in HEK 293 cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

• 同義詞: CD31 antigen, EndoCAM, Platelet endothelial cell adhesion molecule
• 分類程式碼代碼: 12352202
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in HEK 293 cells
• 化驗: ≥97% (HPLC); ≥97% (SDS-PAGE)
• 形狀: lyophilized
• 效力: 1.0-1.5 μg/mL ED₅₀
• 分子量: 80.0-95.0 kDa
• 包裝: pkg of 50 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: P16284
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... PECAM1(5175)

描述

一般說明

PECAM1 (platelet and endothelial cell adhesion molecule 1), a member of the Ig (immunoglobulin) superfamily, is a multifunctional vascular cell adhesion and signaling molecule. It is a surface molecule found on circulating leukocytes, endothelial cells, and platelets. It is a glycoprotein with a molecular weight of 130kDa, and is composed of a single-chain of six Ig-like extracellular homology domains (total 574-residues), a transmembrane portion of 19 residues, and a cytoplasmic tail containing 118 amino acids. This gene is localized to human chromosome 17q23, and is a 75kb gene composed of 16 exons.
Monomeric glycosylated PECAM-1 migrates at an apparent molecular weight of approximately 80.0-95.0kDa by SDS-PAGE analysis under reducing conditions.

生化／生理作用

PECAM1 (platelet and endothelial cell adhesion molecule 1) forms an essential part of the endothelial cell intercellular junctions in vascular beds. Its extracellular domain is involved in homo- and heterophilic interactions, and its cytosolic domain is involved in the transduction of a variety of cellular signaling. It controls vascular inflammatory response as it functions as both pro-inflammatory and anti-inflammatory molecule. Its capacity for hemophilic interactions renders it essential for leukocyte transmigration, interendothelial cell bindings, and vascular permeability regulation. This molecule is thought to be involved in the pathogenesis of various disorders such as thrombosis, cardiovascular disease, inflammation, and cancer.

序列

ENSFTINSVD MKSLPDWTVQ NGKNLTLQCF ADVSTTSHVK PQHQMLFYKD DVLFYNISSM KSTESYFIPE VRIYDSGTYK CTVIVNNKEK TTAEYQLLVE GVPSPRVTLD KKEAIQGGIV RVNCSVPEEK APIHFTIEKL ELNEKMVKLK REKNSRDQNF VILEFPVEEQ DRVLSFRCQA RIISGIHMQT SESTKSELVT VTESFSTPKF HISPTGMIME GAQLHIKCTI QVTHLAQEFP EIIIQKDKAI VAHNRHGNKA VYSVMAMVEH SGNYTCKVES SRISKVSSIV VNITELFSKP ELESSFTHLD QGERLNLSCS IPGAPPANFT IQKEDTIVSQ TQDFTKIASK SDSGTYICTA GIDKVVKKSN TVQIVVCEML SQPRISYDAQ FEVIKGQTIE VRCESISGTL PISYQLLKTS KVLENSTKNS NDPAVFKDNP TEDVEYQCVA DNCHSHAKML SEVLRVKVIA PVDEVQISIL SSKVVESGED IVLQCAVNEG SGPITYKFYR EKEGKPFYQM TSNATQAFWT KQKASKEQEG EYYCTAFNRA NHASSVPRSK ILTVRVILAP WK

外觀

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable