SRP3030

ENA-78 (8-78) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 同義詞: CXCL5, Epithelial Neutrophil Activating Peptide-78, LIX (murine)
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 效力: 10.0-100.0 ng/mL
• 分子量: 7.8 kDa
• 包裝: pkg of 20 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: P42830
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... CXCL5(6374)

描述

一般說明

ENA-78 (epithelial cell-derived neutrophil activating peptide-78) is a CXC chemokine that signals through the CXCR2 receptor. It is expressed in monocytes, platelets, endothelial cells, and mast cells. ENA-78 is a chemoattractant for neutrophils. The murine homolog of ENA-78 is called LIX. Three N-terminal truncated variants of human ENA-78; ENA 5-78, ENA 8-78, ENA 9-78, contain 74, 71, and 70 amino acid residues, respectively, possess increased biological activity. ENA-78 contains the four conserved cysteine residues present in CXC chemokines, and also contains the ‘ELR′ motif common to CXC chemokine that bind to the CXCR1 and CXCR2 receptors. Recombinant human ENA-78 is a 7.8 kDa protein consisting of 71 amino acid residues. The ENA-78 gene is mapped to human chromosome 4q13.3.

生化／生理作用

ENA-78 (epithelial cell-derived neutrophil activating peptide-78) gene expression is associated with sepsis, atherosclerosis, COPD (chronic obstructive pulmonary disease), psoriasis and gastrointestinal inflammation. ENA-78 is angiogenic factor and is associated with cancer progression. Upregulation of ENA-78 gene is observed in non‐small‐cell lung cancer, head, neck, gastric, pancreatic, colorectal, prostate and lung cancer squamous cell carcinoma.

ENA-78 is a CXC chemokine that signals through the CXCR2 receptor. It is expressed in monocytes, platelets, endothelial cells, and mast cells. Recombinant human ENA-78 is a 7.8 kDa protein consisting of 71 amino acid residues.

序列

LRELRCVCLQ TTQGVHPKMI SNLQVFAIGP QCSKVEVVAS LKNGKEICLD PEAPFLKKVI QKILDGGNKE N

外觀

Lyophilized with no additives.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable