SRP0734

PIP4K2B, Active

PRECISIO^® Kinase, recombinant

• 分類程式碼代碼: 12352202
• NACRES: NA.27

屬性

• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: 70-95% (SDS-PAGE)
• 形狀: frozen liquid
• 比活性: 4500-6000 nmol/min·mg (ADP-Glo)
• 分子量: ~73 kDa
• 濃度: 0.1 μg/μL
• 登錄號: NM_003559
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PIP4K2B(8396)

描述

應用

Useful for kinase assay.

生化／生理作用

Recombinant full-length human PIP4K2B was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. PIP4K2B (phosphatidylinositol-5-phosphate 4-kinase, type II, beta) catalyzes the phosphorylation of phospha-tidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. PIP4K2B is a member of the phosphatidylinositol-5-phosphate 4-kinase family. PIP5K2B interacts specifically with the juxtamembrane region of the p55 TNF receptor and that PIP5K2B activity is increased in mammalian cells by treatment with TNF-alpha ⁽¹⁾. PIP5P4KB plays a role in determining insulin sensitivity and adiposity ⁽²⁾.

重構

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

儲存和穩定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法律資訊

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable