SRP0729

PCTK3 (CDK18)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: 70-95% (SDS-PAGE)
• 形狀: frozen liquid
• 比活性: 35-55 nmol/min·mg (radiometric)
• 分子量: ~64 kDa (Cyclin Y); ~76 kDa (PCTK3)
• 濃度: 0.1 μg/μL
• 登錄號: BC011526; NM_145012
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... CCNY(219771) , CDK18(5129)

描述

應用

Useful for kinase assay and Western blot.

生化／生理作用

Full-length recombinant human PCTK3 (CDK18) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 ⁽¹⁾. PCTK3 is expressed in a variety of human cell lines and tissues ⁽²⁾. Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer′s disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.

重構

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

儲存和穩定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法律資訊

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable