SRP0722

KRAS (Mature Form), Active

PRECISIO^® Kinase, recombinant

• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 重組細胞: expressed in E. coli
• 化驗: 70-95% (SDS-PAGE)
• 形狀: frozen liquid
• 比活性: 2.0-3.5 nmol/min·mg (GTPase-Glo)
• 分子量: ~23 kDa
• 濃度: 0.1 μg/μL
• 登錄號: NM_033360
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... KRAS(3845)

描述

應用

Useful for enzyme assay and Western blot.

生化／生理作用

Recombinant human KRAS (2-186) was expressed in E.coli cells using an N-terminal His tag. The RAS gene superfamily encodes a group of closely related 21,000 dalton (p21) proteins with special affinity for guanine nucleotides (GTP). RAS and several other cellular proteins with similar biochemical properties are collectively known as G-proteins and they play key roles in a wide variety of cellular activities, including cell growth, differentiation, secretion, and protein trafficking ⁽¹⁾. There are three forms of RAS gene in cells termed H-RAS, N-RAS, and K-RAS. RAS proteins play a direct causal role in human cancer and in other diseases. Mutant H-RAS, N-RAS, and K-RAS occur in varying frequencies in different tumor types ⁽²⁾. Other members of the RAS superfamily may also contribute to cancer.

重構

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

儲存和穩定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法律資訊

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS08,GHS07
• 訊號詞: Danger
• 危險聲明: H315,H319,H360D
• 防範說明: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危險分類: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable