SRP0339

Ceramide Kinase FLAG tag active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥40% (SDS-PAGE)

• 同義詞: CERK, LK4, hCERK
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: ≥40% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 60 kDa
• 包裝: pkg of 10 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: 0.4 mg/mL
• NCBI登錄號: NM_022766
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... CERK(64781)

描述

一般說明

Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.

Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化／生理作用

Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.

單位定義

One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable