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Key Documents

SRP0277

Sigma-Aldrich

PDE10A1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

同義詞:

HSPDE10A, Phosphodiesterase 10A1, cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥60% (SDS-PAGE)

形狀

aqueous solution

比活性

≥2851 pmol/min-μg

分子量

116 kDa

包裝

pkg of 10 μg

濃度

>0.02 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... PDE10A(10846)

一般說明

Human PDE10A1 (GenBank Accession No. NM_006661), full length with N-terminal GST tag, MW = 116 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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