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SRE0019

Sigma-Aldrich

三磷酸腺苷双磷酸酶 来源于马铃薯

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

同義詞:

腺苷5′-三磷酸酶, 腺苷5′-二磷酸酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.32

品質等級

種類

Grade VII

形狀

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

成份

Protein, 25-60%

溶解度

H2O: soluble 1.0 mg/mL

應用

diagnostic assay manufacturing

異物活動

Acid Phosphatase ≤2% of base activity

儲存溫度

−20°C

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應用

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶:一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应:ATP → ADP+Pi → AMP+2Pi

生化/生理作用

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

單位定義

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

外觀

Partially purified, lyophilized powder containing potassium succinate buffer salts.

準備報告

按 ATP 酶单位销售。

其他說明

该等级具有低 ATP 酶/ADP 酶比率。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Kettlun, A., et al.
Phytochemistry, 21, 551-551 (1982)
Differentiation of platelet-aggregating effects of human tumor cell lines based on inhibition studies with apyrase, hirudin, and phospholipase.
E Bastida et al.
Cancer research, 42(11), 4348-4352 (1982-11-01)
David Riewe et al.
Plant physiology, 147(3), 1092-1109 (2008-05-16)
Apyrases hydrolyze nucleoside triphosphates and diphosphates and are found in all eukaryotes and a few prokaryotes. Although their enzymatic properties have been well characterized, relatively little is known regarding their subcellular localization and physiological function in plants. In this study

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