SML3867

BI, Broccoli Fluorophore

≥98% (HPLC)

• 同義詞: (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one, (Z)-3-((1H-Benzo[d]imadazol-4-yl)methyl)-5-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-3,5-dihydro-4H-imidazol-4-one, BI, DFHBI-BI
• 經驗公式（希爾表示法）: C₁₉H₁₄F₂N₄O₂
• CAS號碼: 2561482-42-4
• 分子量：: 368.34
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: powder
• 顏色: white to beige
• 溶解度: DMSO: 2 mg/mL, clear
• 儲存溫度: room temp

描述

生化／生理作用

Cell-permeable DFHBI derivative that selectively binds to Broccoli aptamer; used for imaging of RNA in living cells.



BI is a cell-permeable DFHBI benzimidazolo (DFHBI-BI) derivative that greatly and selectively promotes and stabilizes Broccoli aptamer folding in vitro and cells with enhanced photostability. BI displays little background in vitro and cells (ϕ=0.00048, ϵ=42,500 M−1 cm−1), but very high fluorescence activation after binding to Broccoli (ϕ=0.669, ϵ=33,600 M−1 cm−1, KD = 51 nM). DFHBI-BI shows negligible cellular toxicity and binds Squash with much weaker affinity than Broccoli. Ex/Em: 470/505 nm.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable