推薦產品
化驗
≥90% (HPLC)
≥90% (TLC)
形狀
solid
分子量
~597 g/mol
溶解度
DMSO: soluble
&epsilon ;(消光係數)
70000 at 490 nm
吸光率
A280/490 nm 0.1
螢光
λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm
適合性
suitable for fluorescence
儲存溫度
−20°C
相關類別
一般說明
CF™488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection conditions, CF™488A is at least as bright as Alexa Fluor™ 488. However, a major advantage of CF™488A over Alexa Fluor™ 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor™ 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF™488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF™488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor™ 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF™488A offers the advantage of less fluorescence "spill-over" in the red channel in multi-color detection applications.
特點和優勢
1. Yields biologically more specific antibody conjugates and has less "spill-over" fluorescence in the red channel than Alexa Fluor™488. 2. Extremely photostable. 3. Highly water-soluble and pH-insensitive.
準備報告
Protect from light
重構
Dissolve in DMSO
法律資訊
This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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