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Key Documents

SAB5801005

Sigma-Aldrich

Alpaca Anti-Mouse IgG(Fcγ) HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

同義詞:

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

alpaca

重組細胞

expressed in E. coli

共軛

horse radish peroxidase (HRP)

抗體表格

affinity purified immunoglobulin

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

lyophilized solid

分子量

14 kDa

物種活性

Alpaca, mouse

技術

ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000

尺寸

0.1 mL

顏色

colorless

同型

VHH

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

Alpaca Anti-Mouse IgG(Fcγ) HRP single domain Antibody is designed to specifically detect the Fc region of mouse IgG. It is a single domain recombinant antibody to mouse IgG(Fcγ fragment specific) coupled to HRP. Alpaca Anti-Mouse IgG(Fcγ) HRP single domain Antibody detects the Fc region of mouse IgG selectively but not with mouse IgM, or the Fab portion of mouse immunoglobulins.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

特異性

Mouse IgG(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

免疫原

Recombinant Alpaca single variable domain secondary nano antibody

包裝

100 μg in Glass bottle

外觀

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

重構

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

儲存和穩定性

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

其他說明

No cross-reactivity with mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

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象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Aquatic Chronic 3 - Skin Sens. 1

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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