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SAB4600463

Sigma-Aldrich

Anti-Rat IgG (H+L), highly cross-adsorbed, CF405M antibody produced in goat

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

CF 405M conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

rat

濃度

~2 mg/mL

技術

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-10 μg/mL
western blot: suitable

螢光

λex 408 nm; λem 452 nm

儲存溫度

−20°C

目標翻譯後修改

unmodified

相關類別

一般說明

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin.(181) Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. There are five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
This product is prepared by labeling highly cross-adsorbed goat anti-rat IgG (H+L) with CF405M. To minimize cross-reactivity, the antibody has been adsorbed against Human, Bovine, Horse, and Rabbit serum. CF405M is spectrally similar to and as bright as Pacific Blue dye but is significantly more photostable than the latter. An additional advantage of CF405M is that it has less spillover in the 525/50 green channel than Pacific Blue dye due to narrower emission peak, making CF405M a superior choice in multi-color detection applications. 

特異性

Binds all rat IgGs (minimal cross-reaction with bovine, horse, human and rabbit serum proteins)

免疫原

rat IgG(H+L)

特點和優勢

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

外觀

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

準備報告

Protect from light. The antibody solution should be gently mixed before use.

法律資訊

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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The Laboratory Rat (2005)
Yegang Chen et al.
Oncology letters, 17(2), 2047-2056 (2019-02-06)
Cell adhesion molecule 1 (CADM1) regulates cell-cell adhesion and an altered expression level is associated with tumorigenesis and progression. The present study assessed CADM1 expression level in 84 bladder tissues to investigate the association with clinicopathological parameters from bladder cancer
The Immunoglobulins: Structure and Function (1998)
Molecular Genetics of Immunoglobulin (1987)
Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)

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