SAB4200846

Anti-LC3C antibody, Mouse monoclonal

clone LC3C-37, purified from hybridoma cell culture, affinity isolated antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 抗體表格: affinity isolated antibody; purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: LC3C-37, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 濃度: ~1 mg/mL
• 技術: immunoblotting: 1-2 μg/mL using whole extracts of HEK-293T cells overexpressing human LC3C.; immunofluorescence: 2.5-5 μg/mL using human HeLa cells treated with Bafilomycin A1 for autophagosomes accumulation
• 同型: IgG1
• UniProt登錄號: Q9BXW4
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MAP1LC3C(440738)

描述

一般說明

At least 16 genes encoding for autophagy (ATG) related proteins that are required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals.6 The Microtubule-associated protein light chain 3 (MAP1LC3), the mammalian homolog of yeast Atg8/Apg8/Aut7, is essential for the formation of autophagosomes.7 In addition, three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues.8The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa). LC3-II is the result of LC3-I cleavage at the C-terminal Glycine by cysteine protease Atg4, which is further modified by the mammalian E1- and E2-like enzymes: Atg7 and Atg3.7 The levels of LC3-II protein correlates with the extent of autophagosome formation.3 Thus, LC3C can be used as an autophagosomal and mitophagy marker.3,7,9-11

特異性

Monoclonal Anti-LC3C antibody specifically human LC3C-I and/or LC3C-II and dose not cross react with human LC3A or LC3B.

免疫原

synthetic peptide corresponding to the N-terminal region of human LC3C, conjugated to KLH

應用

The antibody may be used in various immunochemical techniques including Immunoblotting and Immunofluorescence. Detection of the LC3C band by Immunoblotting is specifically inhibited by the immunogen.

生化／生理作用

Macroautophagy, usually referred to as an autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, called autophagosomes, and subsequently delivered to the lysosome for degradation and recycling.1,2 Although autophagy is a constitutive cellular event, it is enhanced under certain conditions such as starvation, hormonal stimulation and drug treatments.3 Autophagy plays an essential role in cellular differentiation, cell death and aging and is required for normal turnover of cellular components during starvation. Defective autophagy process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections.4,5 Autophagy pathway is evolutionary conserved in all eukaryotic cells.

外觀

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

儲存和穩定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg